Florida Bureau of Public Health laboratories
Hepatitis A Antibody (HAVAb)
The hepatitis A antibody (HAVAb) test provides results for hepatitis A utilizing a chemiluminescent microparticle immunoassay (CMIA) testing method.
Analyte(s)
Hepatitis A IgG
Test Code
0352
Testing Location
Testing Department
Serology
Pre-approval Required?
None
Required Forms
BPHL Test Requisition, DH1847
(not needed if orders are placed via electronic order)
Supply Ordering
Supplies may be requested from your servicing laboratory. Supplies ordered from BPHL should only be used for specimen sent to the BPHL for testing.
Turn Around time
4 business days after receipt at the laboratory
Minimum Volume
At least 2 mL serum
Collection Media
“Tiger” or “yellow” top SST tube
Specimen Sources
Serum
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB, and the date of collection.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
Centrifuge the SST within 8 hours of collection.
Storage Conditions
Specimens may be stored for up to 8 days after collection at 2-8° C.
If testing will be delayed more than 8 days after collection, remove serum from the clot, red blood cells, or separator gel and store at -20° C or colder. Avoid more than 3 freeze/thaw cycles.
Packaging and Shipping
Ship in a cooler with ice packs at 2-8° C to be received at the laboratory no more than 8 days from the collection date.
Specimens stored frozen should be shipped frozen to avoid additional freeze/thaw cycles.
unsatisfactory specimen
1) Incorrect source or incorrect collection device was used
2) Specimen was not labeled with 2 unique patient identifiers
3) Information on specimen does not match requisition/test order
4) Grossly hemolyzed
5) Leaked in transit
7) No specimen received with test order
9) Specimen not centrifuged
10) Quantity not sufficient (QNS)
11) Specimens that exceed the storage limitations listed above.
12) Specimens received at 2-8° C and greater than 8 days after collection will be cancelled.
Interferences and Limitations
1) Specimens containing low antibody concentrations (near the cutoff) assayed after a freeze/thaw may exhibit elevated values that may be false positive.
2) Specimens from patients who have received preparations of mouse monoclonal antibodies for diagnosis or therapy may contain human anti-mouse antibodies (HAMA). Specimens containing HAMA may show either falsely elevated or depressed values.
3) Specimens from individuals with anti-E. coli, anti-CMV, or hemodialysis patients may cross-react.
4) Patients routinely exposed to animals or to animal serum products can be prone to interference and anomalous results may be observed
Reference Range
Nonreactive
Result Indicator
Reactive: IgG anti-HAV detected. Individual is considered immune to HAV
infection.
Nonreactive: IgG Anti-HAV not detected. Individual is considered not immune
to HAV infection
Reference lab
None
Reflex testing
None