Florida Bureau of Public Health laboratories

Mycology Culture for Identification

Candida auris ID is an FDA approved test that utilizes Matrix Assisted Laser Desorption-Ionization Time of Flight Spectrometry (MALDI-TOF) to confirm the identification of Candida spp.

Analyte(s)

Candida spp., yeast isolates identified by submitter as Candida auris

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Test Code

3500

Testing Location

Jacksonville Laboratory

Testing Department

Mycology

Pre-approval Required?

No

Required Forms

BPHL Test Requisition, DH1847
(not needed if orders are placed via electronic order)

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Minimum Volume

Cultures requested

Turn Around time

3-4 business days after receipt at the laboratory.

Supply Ordering

Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.

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Collection Media

Pure isolates cultured on Agar slants

Specimen Sources

Any clinically relevant sources: axilla/groin skin swabs, sputum, blood, tissue, bronchial wash, urine, lesions/wounds, etc.

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, Ex: Name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

Duplicate samples collected within two (2) months will not be tested.

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Storage Conditions

If needed, specimen can be stored at 15-30°C for preferably up to 48 hours after growth.

Packaging and Shipping

Specimens should be shipped at 15-30°C on a nutrient agar slant preferably within 48 hours of growth. Paperwork should be included but separated from the specimen in case of leaks.

unsatisfactory specimen

No patient identification on the sample.
Samples broken or leaked in transit.
Swabs or other unapproved clinical specimens.
Sabouraud Dextrose Agar plates.
Samples in other type of media.

Interferences and Limitations

Contaminated media.

Non-viable, mixed cultures may not yield reportable results

Reference Range

Negative (Not Detected)

Result Indicator

Qualitative: confirmation of Candida auris, Candida duobushaemulonii, Candida haemulonii

Indeterminate or Indeterminate due to equivocal – cannot tell if negative or positive OR invalid – did not return any result twice. All Indeterminates should submit a new sample and re-order test. 

Reference lab

ARLN TN (Antibiotic resistance Laboratory Network Regional Lab, Tennessee) and CDC

Reflex testing

None