Florida Bureau of Public Health laboratories
Enterovirus
BPHL has internally validated (LDT) a qualitative polymerase chain reaction (PCR) enterovirus sequencing test utilizing the sanger sequencing method.
Analyte(s)
enteroviruses (EV)
Test Code
1806
Testing Location
Testing Department
Virology
Pre-approval Required?
Yes. Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.
Required Forms
BPHL Test Requisition, DH1847
Include all required fields, who authorized the testing, onset date, collection date, travel history, and symptoms.
Turn around Time
7-30 business days after receipt at the laboratory.
Minimum Volume
.2 mL
Unsatisfactory specimen
1. Swabs with calcium alginate or cotton tips or with wooden shafts
2. Quantity not sufficient (QNS)
3. Specimen was not labeled with at least 2 unique patient identifiers
4. Information on specimen does not match requisition/test order
5. Specimen leaked in transit
6. Specimen was received out of the acceptable temperature range
Collection Media
Nasal, throat, or rectal swab: Dacron swab in viral transport media (VTM).
Acute Feces, CSF, pericardial fluid, or nasal, throat, or bronchial washing: in sterile container, no preservatives.
Specimen Sources
Nasal, throat, or rectal swab
Acute Feces
CSF
Pericardial fluid
Nasal, throat or bronchial washing
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB, and the date of collection.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
N/A
Storage Conditions
Store specimens at 2-8° C or frozen at less than or equal to -20° C until shipment.
Packaging and Shipping
Specimens must be shipped overnight at 2-8° C or frozen on dry ice at less than or equal to -20° C. If you store the sample frozen, it must be shipped frozen to avoid a freeze thaw cycle.
You must select “Saturday delivery” on packages shipped on Friday.
Couriered samples must be transported in a cooler with frozen ice packs to arrive at the laboratory between 2-8° C. Laboratory staff will check the temperature of the cooler upon arrival before accepting specimens.
Interferences and Limitations
PCR inhibitors
Reference Range
No virus detected
Result Indicator
Name of virus detected
No virus detected
This test is intended for public health purposes only and therefore should not be used for the diagnosis, treatment, or assessment of patient health or management.
Reference lab
CDC, if needed
Reflex testing
None