Florida Bureau of Public Health laboratories
CNS PAnel: CSF
Arbovirus IgG is a lab developed test.
Analyte(s)
Eastern equine encephalitis (EEE), St. Louis encephalitis (SLE), Enterovirus (EV), West Nile (WNV), and herpes (HSV) type 1/2
Test Code
WNV: 1508, EEE: 1502, SLE: 1506, EV: 1810, HSV: 0840
Testing Location
Testing Department
Virology
Pre-approval Required?
Yes.
Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.
Required Forms
BPHL Test Requisition, DH1847
Include all required fields, who authorized the testing, onset date, collection date, travel history, and symptoms.
Turn Around time
7-21 business days after receipt at the laboratory
Test note
If this analysis is selected, regardless of the test code entered, the laboratory will determine which analytes to run based on the current algorithm and the patient’s medical history.
Minimum Volume
1 -2 mL CSF
Collection Media
Labeled sterile container
Specimen Sources
CSF
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.
The collection date and time if submitting multiple specimens.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
N/A
Storage Conditions
Store specimens at 2-8° C or frozen at -20° C or lower until time of shipment.
Packaging and Shipping
Specimens should be shipped on frozen ice packs at 2-8° C or frozen on dry ice at -20° C or lower. It the specimens were stored frozen, then they should be shipped frozen to avoid a freeze thaw cycle.
Separate specimens into different bags to help prevent cross contamination.
Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.
unsatisfactory specimen
1) Requisition form was not fully completed with all required fields
2) No patient identification on specimen tube
3) Patient identifiers on the specimen do not match the requisition or test order
4) Specimen damaged or leaked in transit
5) Specimen was received outside the required temperature range
6) Specimen was received hemolyzed
Interferences and Limitations
PCR inhinbitors
Reference Range
Negative: Not detected
Result Indicator
Positive/name of virus isolated/detected
Negative/no virus isolated/detected
Equivocal
Reference lab
CDC if needed
Reflex testing
None