Florida Bureau of Public Health laboratories
Human Adenovirus
BPHL has internally validated (LDT) a qualitative polymerase chain reaction (PCR) human adenovirus typing test to indicate the type of human adenovirus present.
Analyte(s)
human adenovirus (HAdV) types 3, 4, 7, 11, 14, 16, and 21
Test Code
HAdV3: 9071, HAdV4: 9072, HAdV7: 9073, HAdV11: 9074, HAdV14: 9075, HAdV16: 9076, and HAdV21: 9077
Testing Location
Testing Department
Virology
Pre-approval Required?
Yes. Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.
Required Forms
BPHL Test Requisition, DH1847
Include all required fields, who authorized the testing, bite history, onset date, collection date, travel history, and symptoms.
Turn around Time
For public health surveillance testing.
Minimum Volume
1 mL
Unsatisfactory specimen
1. Swabs with calcium alginate or cotton tips or with wooden shafts
2. Quantity not sufficient (QNS)
3. Specimen was not labeled with at least 2 unique patient identifiers
4. Information on specimen does not match requisition/test order
5. Specimen leaked in transit
6. Specimen was received out of the acceptable temperature range
Collection Media
Dacron swab in viral transport media (VTM) or universal transport media (UTM)
Sterile container
Specimen Sources
Upper or lower respiratory swabs
Nasal aspirate/wash
Bronchoalveolar lavage
Tracheal aspirate
Sputum
Autopsy samples
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB, and the date of collection.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
N/A
Storage Conditions
Store specimens at 2-8° C or frozen at less than or equal to -20° C until shipment.
Packaging and Shipping
Specimens must be shipped overnight at 2-8° C or frozen on dry ice at less than or equal to -20° C. If you store the sample frozen, it must be shipped frozen to avoid a freeze thaw cycle.
You must select “Saturday delivery” on packages shipped on Friday.
Couriered samples must be transported in a cooler with frozen ice packs to arrive at the laboratory between 2-8° C. Laboratory staff will check the temperature of the cooler upon arrival before accepting specimens.
Interferences and Limitations
N/A
Reference Range
No virus detected
Result Indicator
Name of virus detected
No virus detected
Reference lab
CDC, if needed
Reflex testing
None