Florida Bureau of Public Health laboratories
Oropouche virus
BPHL has internally validated (LDT) a qualitative polymerase chain reaction (PCR) oropouche test utilizing the CDC protocol to detect oropouche virus.
Analyte(s)
Oropouche virus
Test Code
1560
Testing Location
Testing Department
Virology
Pre-approval Required?
Yes. Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.
Required Forms
BPHL Test Requisition, DH1847
Include all required fields, who authorized the testing, bite history, onset date, collection date, travel history, and symptoms.
Turn around Time
3-10 business days after receipt at the laboratory.
Minimum Volume
1 mL of serum, 3 – 5mL is preferred
Unsatisfactory specimen
1. Quantity not sufficient (QNS)
2. Specimen was not labeled with at least 2 unique patient identifiers
3. Information on specimen does not match requisition/test order
4. Specimen leaked in transit
5. Specimen was received out of the acceptable temperature range
Collection Media
Serum: red, gold, or tiger top serum separator (SST)
Specimen Sources
Acute serum
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB, and the date of collection.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
Serum should be collected less than 14 days (preferably less than 7 days), post onset of symptoms or exposure
Storage Conditions
Store specimens at 2-8° C or frozen at less than or equal to -20° C until shipment.
Packaging and Shipping
Specimens must be shipped overnight at 2-8° C or frozen on dry ice at less than or equal to -20° C. If you store the sample frozen, it must be shipped frozen to avoid a freeze thaw cycle.
You must select “Saturday delivery” on packages shipped on Friday.
Couriered samples must be transported in a cooler with frozen ice packs to arrive at the laboratory between 2-8° C. Laboratory staff will check the temperature of the cooler upon arrival before accepting specimens.
Interferences and Limitations
Interpretation of rRT-PCR test results must account for the possibility of false-negative results. False-negative results can arise from: poor sample collection, degradation of viral RNA during shipping or storage, specimen collection conducted prior to symptom onset, specimen collection after nucleic acid can no longer be found in the patient
Reference Range
No virus detected
Result Indicator
Name of virus detected
No virus detected
Reference lab
CDC, if needed
Reflex testing
Other arboviruses and/or arbovirus serological testing if needed.