Florida Bureau of Public Health laboratories
Referred isolate for Identification of Acid-Fast Bacilli
The TB drug susceptibilities test is used to determine susceptibility results for 12 anti-tuberculosis drugs (commonly known as first and second-line drugs by MIC (minimum inhibitory concentration) utilizing the Sensititre method.
Analyte(s)
Determines susceptibility results for rifampin, streptomycin, rifabutin, isoniazid, ethambutol, capreomycin, moxifloxacin, amikacin, p-aminosalicylic acid, cycloserine, levofloxacin, and linezolid
Test Code
3300
Testing Location
Testing Department
Mycobacteriology (TB)
Pre-approval Required?
Yes, for out-of-state submissions
Required Forms
BPHL Test Requisition, DH1847
(not needed if orders are placed via electronic order)
Minimum Volume
At least 2 mL of liquid media
Turn Around time
3-10 weeks after receipt at the laboratory
Supply Ordering
Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.
Collection Media
Pure culture identified as Mycobacterium tuberculosis complex (MTBC), growth on solid media preferred, but liquid cultures are acceptable.
Do not send Petri dishes.
Specimen Sources
Any clinically relevant specimen source is acceptable
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.
Information on the specimen must match the requisition and/or test order.Â
Special SPecimen Preparation
Request submission of isolates on solid media whenever possible.
Storage Conditions
Store specimens refrigerated at 2 – 8° C until time of shipment.
Packaging and Shipping
Specimens can be shipped within the 2-28° C temperature range,
Prefer that cultures are shipped in metal cans as primary containers to avoid breakage. Separation of specimens if submitting more than one isolate, to prevent cross-contamination.
Follow all shipping regulations.
unsatisfactory specimen
1) No patient identification on specimen tube
2) No specimen observed in specimen container
3) Specimen damaged or leaked entirely in transit
4) Samples cross contaminated due to sample leaking in transit
Interferences and Limitations
Mixed cultures, mycobacterial blood culture tubes submitted – presence of blood in the tube may interfere with identification methods and require subculture which will delay reporting of test results.Â
Reference Range
Unique values for each drug, available on request.
Result Indicator
Susceptible
Intermediate
Resistant
No interpretation
Reference lab
CDC Â
Reflex testing
None