Florida Bureau of Public Health laboratories
Hepatitis B Panel
The hepatitis chronic panel consists of three FDA-approved hepatitis B tests offered at BPHL to provide a range of results for hepatitis B utilizing a chemiluminescent microparticle immunoassay (CMIA) testing method.
Analyte(s)
Hepatitis B surface antigen, hepatitis B surface antibody qualitative and quantitative, hepatitis B core antibody (IgG + IgM)
Testing Location
Testing Department
Serology
Pre-approval Required?
None
Required Forms
BPHL Test Requisition, DH1847
(not needed if orders are placed via electronic order)
Supply Ordering
Supplies may be requested from your servicing laboratory. Supplies ordered from BPHL should only be used for specimen sent to the BPHL for testing.
Turn Around time
Four business days after receipt at the laboratory.
Minimum Volume
At least 2mL serum
Collection Media
“Tiger” or “yellow” top SST tube
Specimen Sources
Serum
Specimen Labeling
Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB, and the date of collection.
Information on the specimen must match the requisition and/or test order.
Special SPecimen Preparation
Centrifuge the SST within 8 hours of collection.
Storage Conditions
Specimens may be stored for up to 6 days after collection at 2-8° C.
If testing will be delayed more than 6 days after collection, remove serum from the clot, red blood cells, or separator gel and store at -20° C or colder. Avoid more than 3 freeze/thaw cycles.
Packaging and Shipping
Ship in a cooler with ice packs at 2-8° C to be received at the laboratory no more than 6 days from the collection date.
Specimens stored frozen should be shipped frozen to avoid additional freeze/thaw cycles.
Unsatisfactory Specimen
1) Incorrect source or incorrect collection device was used
2) Specimen was not labeled with two unique patient identifiers
3) Information on specimen does not match requisition/test order
4) Grossly hemolyzed
5) Leaked in transit
7) No specimen received with test order
9) Specimen not centrifuged
10) Quantity not sufficient (QNS)
11) Specimens that exceed the storage limitations listed above.
12) Specimens received at 2-8° C and greater than 6 days after collection will be cancelled for HBsAg
13) Specimens received at 2-8° C and greater than 7 days after collection will be cancelled for all tests
Interferences and Limitations
See individual test menu pages for each test code under the hepatitis B panel
Reference Range
See individual test menu pages for each test code under the hepatitis B panel
Result Indicator
See individual test menu pages for each test code under the hepatitis B panel
Reference lab
None
Reflex testing
If HBsAg is reported as reactive, the specimen needs confirmation and will reflex to test code 0305, hepatitis B surface antigen confirmation