Florida Bureau of Public Health laboratories

Aerobic bacteria culture – HAI/AR Isolate

The HAI test is performed for carbapenamase producing organisms for organism identification and is confirmed by MALDI-TOF. mCIM (phenotypic), CARBA5 (gene markers), and Etest (antibacterial suscepbility testing).

Analyte(s)

Aerobic bacteria

Test Code

2910

Testing Location

Jacksonville Laboratory

Testing Department

Microbiology

Pre-approval Required?

No

Required Forms

BPHL Test Requisition, DH1847
Make sure all required fields are complete and the NPI number is listed

View Forms

Minimum Volume

N/A

Turn Around time

5 business days after receipt at the laboratory.

Supply Ordering

Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.

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Collection Media

Nutrient agar slant

Specimen Sources

Any clinically relevant specimen sources.

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

Isolates from the same patient received within 2 months of previous submission will be cancelled.

Storage Conditions

Store specimens at 15 – 30o C until time of shipment. Specimens must be shipped to the laboratory within 48 hours of growth.

Packaging and Shipping

Specimens should be shipped at 15 – 30o C to specimens must be shipped to the laboratory within 48 hours of growth.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) Isolates from the same patient received within 2 months of previous submission will be cancelled.
2) No patient identification on specimen tube.
3) Patient identifiers on the specimen do not match the requisition or test order.
4) Specimen damaged or leaked in transit.

Interferences and Limitations

Non-viable, mixed cultures may not yield reportable results

Reference Range

Negative

Result Indicator

Positive: Identification in culture of bacteria presumptively determined to be resistant to carbapenems

Negative

Reference lab

ARLN TN (Antibiotic resistance Laboratory Network Regional Lab, Tennessee) and CDC

Reflex testing

Whole Genome Sequencing (WGS)