Florida Bureau of Public Health laboratories

Anaerobic Culture

Anaerobic culture organism identification utilizes culture, MALDI-TOF, and 16S RNA gene DNA sequencing to identify bacteria organisms.

Analyte(s)

Anerobic bacteria

Test Code

2500

Testing Location

Jacksonville Laboratory

Testing Department

Microbiology

Pre-approval Required?

No

Required Forms

BPHL Test Requisition, DH1847
(not needed if orders are placed by electronic order)

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Supply Ordering

Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.

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Turn Around time

7 – 28 business days after receipt at the laboratory

Minimum Volume

Not applicable

Collection Media

Anaerobic transport medium

Specimen Sources

Whole blood.

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

None

Storage Conditions

Store specimens at 15 – 30o C until time of shipment. Specimens must arrive at the laboratory within 4 days of collection.

Packaging and Shipping

Specimens should be shipped at 15 – 30o C to arrive at the laboratory within 4 days of collection, on appropriate transport medium.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) Specimens submitted on media without an anaerobic atmosphere
2) No patient identification on specimen tube
3) Patient identifiers on the specimen do not match the requisition or test order
4) Specimen damaged or leaked in transit

Interferences and Limitations

Non-viable, mixed cultures may not yield reportable results.

Reference Range

Negative: Not detected

Result Indicator

Positive: Culture identification

Negative: No pathogen detected

Reference lab

CDC

Reflex testing

Whole Genome Sequencing (WGS)