Florida Bureau of Public Health laboratories

CARBA5 Gene expression

Analyte(s)

Carbapanemase producing organisms gene expression (OXA-48, IMP, KPC, NDM, and VIM) 

Test Code

2907

Testing Location

Jacksonville Laboratory

Testing Department

Microbiology

Pre-approval Required?

No

Required Forms

BPHL Test Requisition, DH1847
(not needed if orders are placed by electronic order)

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Supply Ordering

Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.

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Turn Around time

5 business days after receipt at the laboratory

Minimum Volume

Bacteria cultured on a nutrient agar slant. 

Collection Media

Bacteria cultured on a nutrient agar slant. 

Specimen Sources

Isolates

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

None.

Storage Conditions

Store specimens at 15 – 30o C until time of shipment. Specimens must arrive at the laboratory within 4 days of collection.

Packaging and Shipping

Specimens should be shipped at 15 – 30o C to arrive at the laboratory within 4 days of collection on appropriate transport medium.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) No patient identification on specimen tube
2) Patient identifiers on the specimen do not match the requisition or test order
3) Specimen damaged or leaked in transit

Interferences and Limitations

Will only be tested if phenotypic test of mCIM is positive. 

Reference Range

Negative

Result Indicator

Positive: Identification of gene expression (OXA-48, IMP, KPC, NDM, and VIM)

Negative

Reference lab

CDC and ARLN TN

Reflex testing

None