Florida Bureau of Public Health laboratories

Enteric Stool Culture

This test determines if the tested bacteria produces or not carbapenemase(s) but does not identify the carbapenemase utilizing modified carbapenem inactivation method (mCIM).

Analyte(s)

Carbapenemase producing bacteria

Test Code

2900

Testing Location

Jacksonville Laboratory

Testing Department

Microbiology

Pre-approval Required?

No

Required Forms

BPHL Test Requisition, DH1847
(not needed if orders are placed by electronic order)

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Supply Ordering

Supplies may be requested from your servicing laboratory. Supplies ordered from the BPHL should only be used for specimen sent to the BPHL for testing.

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Turn Around time

5 business days after receipt at the laboratory

Minimum Volume

Not applicable

Collection Media

Pure isolates sent on nutrient agar slant or appropriate media

Specimen Sources

Pure isolate with resistance to carbapenems as detected by phenotypic testing.

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

HAI isolate specimen will not be tested if a duplicate specimen is received within 1 month of the previous submission.

Storage Conditions

Store specimens at 15 – 30o C until time of shipment. Specimens must arrive at the laboratory within 4 days of collection.

Packaging and Shipping

Specimens should be shipped at 15 – 30o C to arrive at the laboratory within 4 days of collection, on appropriate transport medium.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) Duplicate specimen received within 1 month of previous submission
2) No patient identification on specimen tube
3) Patient identifiers on the specimen do not match the requisition or test order
4) Specimen damaged or leaked in transit

Interferences and Limitations

Non-viable, mixed cultures may not yield reportable results

Reference Range

Negative: Not detected

Result Indicator

Positive: Detection of carbapenemase-producing organism

Negative: Not detected

Reference lab

CDC or ARLN TN

Reflex testing

CARBA5 gene expression