Florida Bureau of Public Health laboratories

Arbovirus SN

Arbovirus PRNT is a lab developed test that measures the ability of antibodies to neutralize viruses.

Analyte(s)

Eastern equine encephalitis (EEE), St. Louis encephalitis (SLE), dengue (DEN), West Nile (WN), Zika (ZIK), and Oropouche (OROV)

Test Code

EEE: 1520, OROV: 1565, WNV & SLE: 1522, ZIK: 1536, or DEN: 1524

Testing Location

Tampa Laboratory

Testing Department

Virology

Pre-approval Required?

Yes.
Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.

Find a CHD

Required Forms

BPHL Test Requisition, DH1847

Include all required fields, who authorized the testing, onset date, collection date, travel history, and symptoms.

View Forms

Turn Around time

7 – 21 business days after receipt at the laboratory

Minimum Volume

0.5 mL

Collection Media

Vacutainer (red stopper) or serum-separator tube (tiger/red – topped tube)

Specimen Sources

Serum: single sample or paired serum*

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

*Paired Sera Collection:
1. First specimen (acute) collected in red top tube 1-3 days after onset of illness. Separate serum and store refrigerated at 2 8° C until second specimen is collected.
2. Second specimen (convalescent) collected in red top tube 10-14 days after first specimen.
3. Ship sera together overnight at 2 – 8° C

Storage Conditions

Store specimens at 2 – 8° C or frozen at -20° C or lower until time of shipment.

Packaging and Shipping

Specimens should be shipped on frozen ice packs at 2 – 8° C or frozen on dry ice at -20° C or lower. It the specimens were stored frozen, then they should be shipped frozen to avoid a freeze thaw cycle.

Separate specimens into different bags to help prevent cross contamination.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) Requisition form was not fully completed with all required fields
2) No patient identification on specimen tube
3) Patient identifiers on the specimen do not match the requisition or test order
4) Specimen damaged or leaked in transit
5) Specimen was received outside the required temperature range

Interferences and Limitations

None

Reference Range

Negative: Not detected

Result Indicator

Positive: Titer and interpretation

Negative: Not detected

Reference lab

CDC if needed

Reflex testing

None