Florida Bureau of Public Health laboratories

Arbovirus IgM, EIA

Arbovirus IgM is a lab developed test.

Analyte(s)

Eastern equine encephalitis (EEE), St. Louis encephalitis (SLE), dengue (DEN), West Nile (WNV), Zika (ZIK), yellow fever (YF), and chikungunya (CHIK)

Test Code

DEN: 1500, WNV: 1508, CHIK: 1534, EEE: 1502, ZIK: 1539, YF: 1509, or SLE: 1506

Testing Location

Jacksonville Laboratory
Tampa Laboratory

Testing Department

Virology

Pre-approval Required?

Yes.
Requires prior approval from regional epidemiology and notification to the testing lab. Contact the local county health department (CHD) to start the process for approval.

Find a CHD

Required Forms

BPHL Test Requisition, DH1847

Include all required fields, who authorized the testing, mosquito bite history, onset date, collection date, travel history, and symptoms.

View Forms

Turn Around time

5-10 business days after receipt at the laboratory

Test note

If this analysis is selected, regardless of the test code entered, the laboratory will determine which analytes to run based on the current algorithm and the patient’s medical history.

Minimum Volume

Serum – minimum 1 mL
3-5 mL (preferred) of whole blood
1-2 mL CSF

Collection Media

Serum: Vacutainer or serum separator tube (red/tiger topped tube)

CSF: collected in a sterile, labeled container

Specimen Sources

Single or Paired sera*
Serum
CSF

Specimen Labeling

Specimen must be labeled with at least two unique patient identifiers, e.g., name and DOB.

Information on the specimen must match the requisition and/or test order. 

Special SPecimen Preparation

*Paired Sera Collection:
1. First specimen (acute) collected in red top tube 1-3 days after onset of illness. Separate serum and store refrigerated at 28° C until second specimen is collected.
2. Second specimen (convalescent) collected in red top tube 10-14 days after first specimen.
3. Ship sera together overnight at 2-8° C

Storage Conditions

Store specimens at 2-8° C or frozen at -20° C or lower until time of shipment.

Packaging and Shipping

Specimens should be shipped on frozen ice packs at 2-8° C or frozen on dry ice at -20° C or lower. It the specimens were stored frozen, then they should be shipped frozen to avoid a freeze thaw cycle.

Separate specimens into different bags to help prevent cross contamination.

Include paperwork with specimens but separate them physically by placing the paperwork in the outer pocket of the biohazard bag.

unsatisfactory specimen

1) Requisition form was not fully completed with all required fields
2) No patient identification on specimen tube
3) Patient identifiers on the specimen do not match the requisition or test order
4) Specimen damaged or leaked in transit
5) Specimen was received outside the required temperature range
6) Specimen was received hemolyzed

Interferences and Limitations

Hemolysis

Reference Range

Negative: Not detected

Result Indicator

Positive

Equivocal

Inconclusive

Negative

Reference lab

CDC if needed

Reflex testing

None